The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR merchandise have been digested and cloned into a pET21a+ vector applying NdeI and XhoI restriction enzymes. A point mutation was released by the fast alter https://dallasxgnr41841.blog5star.com/26895577/gne-617-for-dummies